Chetan Sharma,
Here is some workarounds comes to my mind depending on what you want to achieve:
If you want to just show a message whenever user entered an existing field data you may use General Condition validation rules on the field which allows you to define a Macro rule and I think with a macro method you can check if the value exist or not. I have never test it but may it worse to give it a go!
https://docs.kentico.com/display/K8/Designing+forms+using+the+Form+builder
But if you want to generate a unique value while adding a record, you may use default value macros and generate for example add to max of that field.
https://docs.kentico.com/display/K8/Reference+-+Field+editor