Could not load signalR

This may be a duplicate question but after lot of tries, I am not able to setup signalR with my Kentico

• Kentico Version: 10
• signalR: 2.4.1 ( Installed Microsoft.AspNet.SignalR via Nuget )

Here are the steps I did:

• Scripts folder is generated on root that contains jquery and signalr js files
• Created following classes in App_Code folder

ChatHub.cs

using System;
using CMS.Membership;
using Microsoft.AspNet.SignalR;

public class ChatHub : Hub
{
    ..code logic..
}

Startup.js

using System;
using System.Threading.Tasks;
using System.Web.Routing;
using Microsoft.Owin;
using Owin;

[assembly: OwinStartup(typeof(Startup), "Configuration")]
public class Startup
{
    public void Configuration(IAppBuilder app)
    {
        app.MapSignalR();
    }
}

DLLs that I can see

• Microsoft.AspNet.SignalR.Core.dll
• Microsoft.AspNet.SignalR.SystemWeb.dll
• Microsoft.Owin.dll
• Microsoft.Owin.Security.dll

I added following scripts on my page

• /Scripts/jquery.signalR-2.4.1.min.js
• /signalr/hubs

Added ;/signalr in urls to ignore in Settings => URLs and SEO => Excluded URLs:

I did not add jquery-1.6.4 from the Scripts folder as jquery-core (1.7) is already added to project

Changes in web.config

<system.webServer>
    <modules runAllManagedModulesForAllRequests="true">
      ...
      ...
    </modules>
    <validation validateIntegratedModeConfiguration="false" />
</system.webServer>    
<appSettings>
     <add key="owin:AutomaticAppStartup" value="false" />
     ...
</appSettings>

Whatever I do, I always get:

GET http://localhost:8081/signalr/hubs net::ERR_ABORTED 404 (Not Found)

I did not exclude AjaxMin.dll otherwise I get errors in AjaxCssMinifier.cs

What am I missing here?

Thanks